Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2016 Vol: 8 Issue: 6

Green synthesis, characterisation and biological activities of silver nanoparticles by using Wrightia tomentosa leaves

Abstract

The present study was aimed at green synthesis of silver nanoparticles (AgNPs) using aqueous leaf extract of an endangered plant Wrightia tomentosa at room temperature. Synthesis of WT-AgNPs was characterized by visual colour change of the reactants due to the reduction of AgNO3 by the plant extracts which also act as capping agents. An intense band of Plasmon resonance was observed at ∼454 nm in UV visible spectrum confirms the formation of WT-AgNPs. Study of the various functional groups of the biomolecules capping the AgNPs was confirmed by FTIR spectrum. XRD analysis reveals the degree of crystallinity and face centered cubic structure of the monophasic AgNPs. In addition, the shapes and nature of the AgNPs was also confirmed by SEM, while SEM-EDX confirmed the presence of elemental silver and TEM studies reveal the shape of the AgNPs. DLS revealed the mean size of the nanoparticles formed and measured 2.9 nm in monodispersed condition. Further the antimicrobial and Minimum Inhibitory Concentration (MIC) potency of the synthesized AgNPs was tested against pathogenic Gram positive, Gram negative bacterial and fungal strains that showed promising activity. Cytotoxicity of the WT-AgNPS was tested against MDA-MB-231, Hela, MCF-7 and OAW-42 cancer cell lines.