Bromeliads are epiphytes which are distributed in subtropical and tropical latitudes of South America. In Ecuador there are about 455 species with inflorescence’s with a unique beauty of intense colors and long lasting, reason has been of great interest in many countries. But human activities such as the introduction of new species, over exploitation and habitat loss have resulted in the negative impact on bromeliads and leads to the extinction of species. Hence, the objective of this research is the implementation of a pilot germplasm bank by an ex situ process of adaptation and development of biotechnological tools such as in vitro culture of cryopreservation and minimal growth for the conservation of the four bromeliads species. Thus the species that have shown positive results with minimal growth of cryopreservation obtained viable and hydrated plants.