Due to fenugreek based on different grown environment has vital differences in quality and physiological efficacy, this study focused on classification and identification of fenugreek seeds from different production areas using Fourier Transform Infrared (FT-IR) Spectroscopy coupled with cluster analysis. Chemometrics fequencies at 1800- 400 cm−1 were exploited for the establishment of all chemometric models after systematic noise reduction, 13-point smoothing, and normalization of raw FTIR data. Results showed that the spectral data of fenugreek seeds from different production areas had many similarities and differences mainly because of the different kinds of material compositions. Therefore, infrared spectroscopy coupled with multivariate analysis is an effective, specific, rapid, nondestructive, and nonpolluting identification method of identifying fenugreek seeds from different production areas. The proposed methodology can also reflect the differences among fenugreek seeds material component contents from different producing areas.