In the last few decades there has been an exponenti al growth in the field of herbal medicine. It is getting popularized in developing and developed cou ntries owing to its natural origin and lesser side effects. The scientific evidence has brought a bout the possibility of utilization of herbal plant in the treatment of fungal and bacterial infections and the development of anti-bacterial and anti-fungal products. In the present study, herbal ointment containing Aloe vera, Neem and Turmeric was formulated and evaluated to study anti bacterial and antifungal activity. The evaluation is done using cup plate method for zone of inhibition and two fold dilution method for MIC (Minimum Inhibitory Concentration).The study sh owed that Aloe ointment is exhibiting broad-spectrum antifungal activity against A. vari es and antibacterial activity against E.coli. The overall experiment showed that Aloe ointment an d Turmeric ointment showed more anti- fungal activity than Neem ointment. Also it was fou nd that ointment containing mixture of Aloe vera, Neem, Turmeric showed prominent antifungal ac tivity than antibacterial activity. This ointment can be used in the treatment of sun burns, rashes, burns, wounds and other skin infections. It can also be used in the treatment of superficial mycosis.