Fucoxanthin is a Xanthophyll. It is found as an accessory pigment in the chloroplasts of brown algae, Sargassum cinereum. The crude pigment was initially screened through TLC and then fucoxanthin was separated and partially purified using silica column chromatography (230 – 400 mesh). The yield of fucoxanthin from Sargassum cinereum was 2.0225mg/g of sample. Fucoxanthin was found to be present in Sargassum cinereum extract, which was detected using UV vis spectrophotometer at 422.90nm and identified using FT-IR, HPLC and GC-MS. The fucoxanthin was later checked with free radicals for its antioxidant property. 60μg/ml of pigment showed 50% of scavenging activity of free radicals.