Melghat region is a rich source of various important medicinal plant species. More than 700 naturalized plant species have been enlisted in the Flora of Melghat. Skin disease, an important health problem found worldwide, especially in developing countries. In recent years there has been a great interest in herbal remedies for the treatment of a number of skin ailments. Medicinal plants are promising source for preparing drugs to cure skin diseases. Fresh flowers of Cassia fistula plant were isolated for various ingredients i.e. terpenoids and phenolics, fats and waxes, alkaloids, quaternary alkaloids and N-oxides. These ingredients were characterized and screened for their antimicrobial activity against certain pathogens by cup plate agar well diffusion method. The pathogenic microbes selected are generally the causative agents for skin diseases. All the ingredients showed moderate to good antipathogenic activity. The result shows that the test plant might be useful in curing the skin ailments.