phellinus igniarius is a filamentous fungi with good anti-cancer effect world-recognized now, which has broad prospects in the field of medicine because it contains a large number of active substances. cdc42 proteins are a class of key proteins in transporting cancer cells, this study clone and express cdc42 protein using molecular biology tools, successfully obtained gene sequence information and protein sequences and then detected their expression in E. coli, it provides some theoretical basis in further to research cdc42 proteins and phellinus igniarius exploitation.