A survey for the presence of anoxygenic photosynthetic bacteria was conducted and the results are analysed in the present study. The organism preferred sucrose and ammonium chloride for maximum biomass production. pH between 5.0 to 6.0 was optimum for the growth of the organism. Rhodopseudomonas palustris MGU001 achieved maximum growth on 10th day of incubation under anaerobic light. Studies on optimization of cultural conditions for production of hydrogen revealed that the maximum hydrogen production took place on 24th hour of incubation. pH of 6 was amenable for the production of hydrogen. More hydrogen production was seen when glutamine and glucose were used as nitrogen and carbon sources respectively.