Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2016 Vol: 8 Issue: 3

Evaluation of the bactericidal efficacy of different dilutions of tincture of iodine on three bacterial reference strains

Abstract

The aim of this study is to evaluate the bactericidal efficacy of a disinfectant (iodine) on three bacterial reference strains which are Pseudomonas aeruginosa (CIPA22); Escherichia coli (CIP 55.30) and Staphylococcus aureus (CECT 59), according to the standard (AFNOR NF T72_150, 1995). The results showed that the Gram-negative Pseudomonas aeruginosa strains (CIPA22) and Escherichia coli (CIP 55.30) are more resistant to iodine. Furthermore, Staphylococcus aureus(CECT 59) is the most sensitive gram-positive strain to this disinfectant. No bactericidal dose has been observed in the dilutions used except for dilution (½) of Staphylococcus aureus(CECT 59). The results of this study were used to classify the disinfectant according to its activity, its vis-à-vis efficiency of Gram negative and positive strains and its bactericidal dose.

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