Fifteen stations from water surfaces were selected in southern of Iraq at Basra city , it was distrib uted on Euphrates river , Shatt al-arab and Main outfall Dr ain River . All water samples were collected from s urface water at 10-15 cm depth . Selenite and total Selenium we re measured by Spectrophotometric method through c omplex formation with Selenium(IV) in acidic medium from Hydrochloric acid , some physical properties of w ater were recorded in these stations . The results of recordi ng have shown the highest concentration of selenite ( 36.0069 μg/l) in station W 3 and lowest concentration( 12.224 μg/l) in station W 5 , while , the highest concentration of total Selenium( 54.413 μ g/l) was recorded in station W 8 and lowest concentration( 17.381 μ g/l) was recorded in station W 5 . Standard deviation of all stations (n=3) for Selenite and total Selenium were calculated, it shows at extent (0.013014 - 0.289165 ), ( 0.073961- 0.477617 ) respectively .