Original Articles: 2015 Vol: 7 Issue: 1
Evaluation of invitro free radical scavenging potential of various extracts of whole plant of Calycopteris floribunda (Lam.)
The present study was aimed to investigate the free radical scavenging potential of various extracts from whole plant of Calycopteris floribunda with the help of two in-vitro antioxidant models were carried out for total antioxidant activity (phosphomolybdic acid method), ferric reducing antioxidant potential (FRAP) assay and estimation of total flavonoids using spectrophotometric methods. Ascorbate was used as standard and positive control for above models. Ethyl acetate extract of Calycopteris floribunda was found to be extremely effective in total antioxidant activity. The IC50 values of ethyl acetate extract of Calycopteris floribunda and ascorbate were found to be 360μg/ml and 410μg/ml respectively. The ethyl acteate extract of Calycopteris floribunda was found more efficient in FRAP assay than that of petroleum ether and methanolic extract. Results indicate that ethyl acteate extract have marked amount of flavonoids, which could be responsible for antioxidant activity. Our findings revealed that ethyl acetate extract of Calycopteris floribunda possess interesting antioxidant activity, which may provide protection against free radicals induced damage to biomolecules. It is also worth noting that these results validate with invitro tests, the therapeutic use of the plant in traditional medicine.