Ibuprofen ((RS)-2-(4-(2-methylpropyl) phenyl) propanoic acid) is a well-known non-steroidal anti-inflammatory drug (NSAIDs) widely used for the treatment of pain, fever and inflammation. This study designed to evaluate if dose of 30mg/kg of Ibuprofen has an antioxidant effect, relative to its analgesic, antipyretic and anti-inflammatory activities. Oxidative stress was induced by intraperetoneal injection of (100 mg/kg) peroxide hydrogen (H2O2), and then a comparative study is made concerning the activities of the antioxidant enzymes catalase (CAT), superoxide dismutase (SOD), gluthation reductase (GR), succinate deshydrogenase (SDH), and index of lipid peroxidation: thiobarbituriquc acid reactive substances (TBARS) determined lipid peroxidation in mice treated with H2O2 accompanied by Ibuprofen; compared to the group treated by L-ascorbic acid + H2O2, These results suggest that Ibuprofen may exert protective effects against oxidative stress damages.