The aim of present study was to evaluate the best possible temperature and humidity affect on germination power of Aegle marmelos seeds Invitro. The study was conducted by placing seeds in desicator, to maintain moisture contents (18.9, 13.8, 6.3 and 4.47 %) and at temperature range of 30o, 20o, 10o, 0o -20o and -196 oC, for 15 days. On sixteenth day germination power of seeds was evaluated by keeping in seed germinator at 30o C / 80 % RH in dark. It was observed that high moisture content (18.9 %) and high temperature (30o) showed maximum germination (about 97%) within shortest time period (about 9 days).