The present study was conducted to investigate the presence of biochemical contents, trace elements, nutritive value evaluation and determinat ion of molecular weight of proteins by SDS- PAGE and phytochemicals detection by HPTLC in the l eaves and 50% hydroethanolic leaf extracts of Ruellia tuberosa L. and Dipteracanthus patulus (Jacq.). The biochemical contents, trace elements, nutritive value were determined by different biochemical methods, trace elements presence was detected by using Atomic Absorption Sp ectroscopy (AAS), while the proteins and phytochemicals were detected by using SDS-PAGE and HPTLC. Ruellia tuberosa L. and Dipteracanthus patulus (Jacq.) leaves confirmed the presence of flavonoids, glycosides, phenols saponins and showed minimum amount of trace element s with moderate nutritive value. Vitamins (E, C), total phenolics, carotenoid content and nut ritive value were found to be greater in the leaves of Ruellia tuberosa L. The protein bands obt ained in the SDS-PAGE was found to be similar for both the plant leaves. Our findings sug gest that leaves of Ruellia tuberosa L. and Dipteracanthus patulus (Jacq.) is endowed with anti oxidant phytochemicals and moderate nutritive value could serve as a base for future dr ugs.