This is the first attempt reported on the antioxidant activities of Heterodermia boryi, Sticta weigelii and Dermatocarpon vellereum.The levels of super oxide dismutase were evaluated in Heterodermia boryi, Sticta weigelii and Dermatocarpon vellereum. Among which SOD levels were found to be higher in Heterodermia boryi (4460 U/g). Catalase was found to be higher in Dermatocarpon vellereum (113.01 U/g). Peroxidase activity was higher in Dermatocarpon vellereum (829.12 U/g). GST level was higher in Sticta weigelii (454.87 U/g). Polyphenol oxidase was estimated in the form of catechol and laccase. COX and LAC levels were higher in Dermatocarpon vellereum (9.973 and 9.33 U/g) and total polyphenol oxidase was found to be higher Dermatocarpon vellereum (19.23 U/g) Ascorbic acid level was higher in Sticta weigelii (24.29 mg/g) Tocopherol were reflected higher in Sticta weigelii (1474 μg/g). Reduced glutathione was found to be higher in Heterodermia boryi (11.63 nano moles/g). ABTS radicals were highly scavenged by Sticta weigelii (36.91%) followed by Dermatocarpon vellereum (35.02%) and Heterodermia boryi (31.81%).DPPH radicals were highly scavenged by Heterodermia boryi (90.66%). The inhibitory effect on lipid peroxidation by the lichens was estimated and was found that Dermatocarpon vellereum (92.26%). Comparatively Heterodermia boryi and Sticta weigelii possessed lesser LPO (94.17% and 85.51% respectively). Inhibition of oxidant induced DNA damage in herring sperm was evaluated, Sticta weigelii greatly inhibited (92.24%).