The use of medicinal plants is old in society. Several studies have been conducted to assess the biological potential of plant species. One of the studied families is Bignoniaceae. This research aimed to evaluate the antimicrobial and cytotoxic activity of Handroanthus ochraceus. The antibacterial activity was determined by the Minimum Inhibitory Concentration (MIC) and to evaluate the samples cytotoxicity was conducted the MTT (3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide) tetrazolium reduction assay. As the results, the plant species has no antibacterial activity against bacterial strains tested, about cytotoxicity, extracts of leaves and twigs varied their toxicity according to tested concentrations. Further researches that aimed other biological activities are required