Tinospora cordifolia is used to treat various diseases in the traditional medicinal system in India. Its chemopreventive potential for cancer was the subject of present study. In this study the anticarcinogenic and antimutagenic activity of T. cordifolia extract was used, in C57 Bl mice and Swiss albino mice respectively. In antimutagenic study, a single application of T. cordifolia extract at a dose of 200, 400 and 600 mg/kg dry weight, 24 hrs prior the i.p. administration of cyclophosphamide (at the 50 mg/kg), significantly prevented the micronucleus formation in bone marrow of mice, in a dose dependent manner. In melanoma assay, C57 Bl mice when received 50% methanolic extract of T. cordifolia at a dose 750 mg/kg body weight for 30 days showed increase in life span and tumor size was significantly reduced as compared to control.