The presente study was conducted to evaluate the anticancer activity of Alphonsea sclerocarpa with a focus of its cytotoxicity on HepG2 cancer cell linesCrude extract was prepared by soxhlet extraction. HepG2 cell lines were assessed by MTT assay after treatment with various concentrations (0.78-500 μg/ml) for 48 h shows IC50 at 199.98 μg/ml indicates dose dependent manner. Further, nuclear morphology changes were carried by DAPI shows changes in morphology, apoptotic body formation. Apoptosis which was further confirmed through increasing nuclear DNA damage. In conclusion, the present findings clearly indicated that Alphonsea sclerocarpa showed dose dependant cytotoxicity.