Thiadiazoles are heterocyclic compounds of importance that exhibit diverse biological properties as anticancer, antibacterial, fungicidal, antiviral, antiparasitic, anticonvulsant, anticoagulant, antidiabetic, anti-Helicobacter pylori, leishmanicidal agents. The biological activity of thiadiazole is mainly due to the unsaturation of the ring system, which leads greater in vivo stability and lack of toxicity. The present study aimed to synthesize 2, 5 substituted 1,3,4 thiadiazole nucleus and evaluates its anti-inflammatory property using cell lines and membrane stabilization methodalong with in vivo models. The compound, 5-(5-(phenyl amino)-1,3,4 thiadiazole-2yl) benzene 1, 2,3 triol was synthesized via microwave irradiation method and the characterization done by TLC,IR,NMR and Mass spectral analysis. It showed anti-inflammatory activity when tested for cycloxygense, lipoxygenase and myeloperoxidase inhibition using RAW 264.7 macrophage cells cultured in Dulbecco’s media. In vivo anti-inflammatory activity of the test drug, thiadiazole derivative was investigated using acute and chronic models of inflammation, carrageenan induced paw edema method and cotton pellet granuloma method respectively.