Antibacterial activity of hexane, petroleum ether, chloroform and methanol extracts obtained from the aerial parts (leaf, flower and stem) of Tridax procumbens was tested against both gram positive (Staphylococcus aureus and Bacillus subtilis) and gram negative (Enterobacter aerogenes) bacteria using the agar well diffusion method. The susceptibility of the test bacteria varies with the types of solvents and plant parts used. The flower posses potent antibacterial activity with 20 mm of zone of inhibition against E. aerogenes in hexane extract whereas the leaf showed antibacterial activity in all the solvents used. The stem exhibited moderate inhibitory effect on the test bacteria. The potentiality of the leaf against the test bacteria as evaluated by minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) indicated the presence of more active compounds in methanol than in hexane extracts. Chloroform extracts was found to be least active, whereas, petroleum ether possessed moderate effect on the test bacteria. These results may suggest the distribution of antibacterial potential in different aerial parts of T. procumbens that can be explored further for the isolation and charachterization of the compound agent in pharmaceutical industries.