Aesculus hippocastanum (family Hippocastanacae) is commonly known as Horse chestnut is rich in many phytochemicals and has many potential medicinal properties. The seeds have been used as an analgesic, antipyretic, narcotic, tonic, and vasoconstrictor. They have been used to treat backache, sunburn, neuralgia, rheumatism, whooping cough and hemorrhoids. They have pharmacological effects such as anti-hyperlipidemic, antiinflammatory, antioxidative effects, anticarcinogenic, and cytoprotective. In this study, we have made an attempt to estimate Epicatechin present in the Horse Chest Nut (HCN) extract by HPTLC technique as it is known for its therapeutic benefits so that further exploration of the Aesculus hippocastanum can serve millions in a variety of disease conditions. The ethanolic extract of Aesculus hippocastanum was chromatographed on silica gel GF254 plates with Toluene: EA: FA: MeOH (3: 3: 0.4: 0.1) as mobile phase. Detection and quantification were performed by densitrometric scanning, at 280 nm. The average recovery of Epicatechin was found to be 0.81 %. The HPTLC technique has provided a good resolution of Epicatechin from other constituents present in the ethanolic extract.