Qualitative and quantitative analysis of ciprofloxacin in drug formulations using RP-HPLC treated and maintained at various physiological pH conditions to evaluate the effect of pH on ciprofloxacin active ingredient on different mode of drug administration. The result in this study gave a clear evidence that there is an effect of pH on drug compound or active ingredient ciprofloxacin. The effect is in both ways qualitative by degradation and quantitative by reduced level of concentration.