A series of newly Atophans (Cinchophen, 2-phenylcinchoninic acid) heterocycles have been synthesized by employing 2-phenylquinoline-4-carboxylic acid (cinchophen) as starting material with the aim of evaluating their antimicrobial activity. The key intermediate 2-phenylquinoline-4-carbohydrazide (3) was smoothly synthesized by reaction of phenylcinchoninic ethyl ester with hydrazine hydrate in ethanol. The structures of synthesized compounds were confirmed on the basis of their elemental analysis and spectral results (IR, 1H and 13C NMR). The synthesized compounds were screened for their bactericidal and fungicidal activities. The preliminary results revealed that some of the compounds exhibited promising antimicrobial activities.