Hibiscus sabdariffa is an annual dicotyledonous herbaceous shrub plant popularly known as “zobo” in Nigeria which is an indigenous edible medicinal plant used in Ayurvedic medicine in India, China and Thailand. The purpose of this studies is to investigate the effect of Hibiscus sabdariffa leaf extract on liver marker enzymes such as AST (aspartate aminotransferase), ALT (alanine aminotransferase) and ALP (alkaline phosphatase), for its hepatoprotective effect in phenobarbitone-induced rats. Phenobarbitone treated rats showed a significant increase (P<0.05) in the levels of circulatory AST, ALT and ALP. These changes were significantly decreased (P<0.05) in rats treated with HSEt and phenobarbitone. These results indicate that HSEt offers hepatoprotection by influencing the levels of liver markers in phenobarbitoneinduced rats and this could be due to its free radical scavenging property and the presence of natural antioxidants.