The present work aimed to evaluate the antioxidant and antiproliferative properties of leaves and stems of the Lebanese Eryngium creticum grown in Bekaa at 1200 m and then to compare the obtained results with those obtained using the same plant grown up at 300 m in order to determine the effects of the altitude on the chemical composition and thus on the biological properties of this plant. Phytochemical screening results show that the two studied parts of E. creticum are rich in a variety of secondary metabolites depending on the used solvent. In addition, an antioxidant activity arises due to phenolic compounds present. Increasing number of RAW264.7 cells showed some anti-inflammatory properties. At the same time, an antiproliferative capacity was observed using MCF-7 and MDA-MB-468 cells. All of these results have been compared with others obtained using the same plant growing at 300 m. Our study showed that the altitude exerts an effect on the chemical composition of the plant and thus on its biological activities.