The present study deals with the production of lipase enzyme from Bacillus sp. isolated from the soil samples from around Bharath University, Chennai, India. One of the parameters in the production of lipase is the speed levels of agitation performed in the bioreactor. Germinated maize oil was used as the main carbon source along with other substrates. Different trials at different ranges of agitation speeds were performed. It was observed that maximum lipase production of 48 IU/ml was achieved at 700 rpm agitation speed.