The present study was conducted during 2012-2013 in the sub-tropical forest of Mizoram. Three study sites representing undisturbed (un-mined area), moderately disturbed (mining 10 years back) and highly disturbed (ongoing mining operation) were selected for detailed investigation. The soil samples were collected seasonally during monsoon and post-monsoon seasons considering top-soil (0-10 cm depth) and sub-soil (10-20 cm depth), for analysis of various soil characteristics namely, moisture content, pH, organic carbon, total nitrogen, and available phosphorus. The findings depicts that soil pH ranged from 5.57 (undisturbed stand) to 6.83 (highly disturbed stand) indicating acidic nature of soil. Soil moisture content was ranged from 31.4 to 18.6 % from undisturbed to the highly disturbed stand. There was sharp decrease in organic carbon from undisturbed (3.64 %) to the highly disturbed (2.08 %) stand. Similarly, total nitrogen decreased from 0.31% to 0.12 % and phosphorus content decreased from 0.32 ppm to 0.11 ppm, from undisturbed to highly disturbed stand. The C:N ratio also decreased from highly disturbed stand (20.0) to the undisturbed (11.28). A similar trend in result was observed for both topsoil and sub-soil. There was significant correlation between various parameters and study sites.