The quality of life on earth is linked undeniably to the overall quality of the environment. Pollution of the biosphere by heavy metals due to industrial, agricultural and domestic activities has created a serious problem for the safe and rational utilization of soil. Industrial inputs and the agronomic application of fertilizers, pesticides and metalcontaminated sewage continue to contribute the metal accumulation in the soil . The pollution of the ecosystem by heavy metals is a real threat to the environment because metals cannot be naturally degraded like organic pollutants and persist in the ecosystem having accumulated in different parts of the food chain. Here effect of copper sulphate on Citrobacter has been discussed on its growth. These findings will be very beneficial to reveal the causes behind soil pollution.