The dipeptidyl peptidase-4 DPP-IV is the major receptor of the new oral treatments for diabetes type II. This work determined the structural similarities of the protein DDP-IV of the rat and the human through the global alignment in three dimensions and the aminoacidic sequence. The parameters of structural difference of identity between two proteins 0.751 RMSD and high percent identity 83.56% does not localize in the active site of the enzyme, concluded that activity in both species are similar. The important results are useful in the area preclinical, clinical and developments of drugs that focus in the treatment of diabetes type II.