Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

header
Reach Us reach to JOCPR whatsapp-JOCPR +44 1625708989
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Original Articles: 2010 Vol: 2 Issue: 6

DNA-binding and Cleavage studies of Imidazole Cobalt (III) Ethylenediamine Complexes

Abstract

Four imidazole Cobalt(III) complexes of the type [ Co(en) 2 (L) 2 ] 3+ (en = ethane 1, 2, diamine, L = imidazole) (1), [Co(en) 2 (L) 2 ] 3+ (L = methylimidazole) (2), [Co(en) 2 (L) 2 ] 3+ (L = 1, 2, dimethylimidazole) (3), [Co(en) 2 (L) 2 ] 3+ (L = ethylimidazole) (4) have been isolated and characterized by Elemental analysis, IR, and 1 H, 13 C NMR spectral methods. The binding of the complexes with calf thymus DNA has been investigate d by absorption, emission spectroscopy, viscosity measurements, DNA melting, and DNA photoc leavage. The spectroscopic studies together with viscosity measurements and DNA meltin g studies support that all the complexes bind to CT DNA(=calf thymus DNA) by groove mode. Co mplexes 3 & 4 bind more avidly to CT DNA than 2 & 1. Noticeably, these complexes were fo und to promote cleavage of plasmid DNA pBR322 with incubation of complexes