The methyl-D-erythritol 4-phosphate pathway (MEP) and Mevalonate Pathway (MVP) is responsible for the biosynthesis of a substantial number of natural compounds of biological and biotechnological importance. In recent years, this pathway has become an obvious target to develop new herbicides, pharmaceuticals and antimicrobial drugs. The numerous secondary metabolites are produced through this pathway which involved in defense response against biological and non-biological agents. Some of them have broad economical functions like improving the interaction between the plant and environment. Andrographolide is one of the principal secondary metabolite of A. paniculata. It has multiple health promoting effects including anti HIV property. In this study we have exogenously applied Jasmonic acid (JA) and Gibberelic acid (GA3) as an elicitor, in variable concentration, to access the effect on andrographolide accumulation and chlorophyll content of A. paniculata. The result indicated that the biosynthesis of andrographolide has enhanced by 31.25% and 56.1% by application of GA3 and JA respectively. Increment in the diterpene content has shown its association with the expression level of hmgr genes in the andrographolide accumulation regulatory cascade.