Original Articles: 2012 Vol: 4 Issue: 3
Development of paptodes for determination of hydrazine in water and its application in biological samples and pharmaceutical tablet
A new disposable chemo-strip is developed for detection and determination of hydrazine. The sensor was constructed by immobilizing vanillin and oxalic acid on TLC (Thin layer chromatography) paper. In this method hydrazine is coupled with vanillin in acidic medium and formed yellow aldazine product. The products are detected by scanner. Changes in RGB values of color spots on TLC strips create a pattern. The obtained pattern was analyzed by “MATLAB software”. Degree of color spot was found to be proportional to the concentration of the tested analyze i.e. hydrazine. The proposed sensor is linear in concentration range of 1.92-6.72μg/ml of hydrazine. Effective intensity, Reproducibility, SD, RSD have been calculated. The reaction time, concentration of reagent, stability of sensor has been optimized. The method has been successfully applied for the determination of hydrazine in waste water, biological and pharmaceutical samples.