Two new, simple and sensitive spectrophotometric methods (A and B) have been developed for the quantitative determination of Mesalamine in bulk drug and pharmaceutical preparations. Method A is based on the condensation of Mesalamine with p-hydroxy benzaldehyde to form schiff’s base, which was an yellow colored chromogen exhibiting λmax at 420 nm. Method B is based on the reaction of Mesalamine with Folin-Ciocalteu phenol’s reagent under alkaline conditions forming a blue colored chromogen exhibiting λmax at 750 nm. Beer’s law is obeyed in the concentration range of 8-28 μg/ml for method A and 4-24 μg/ml for method B. These methods were extended to pharmaceutical formulations and there was no interference from any common excepients. The results of analysis have been validated statistically and by recovery methods.