A new RP-HPLC method was developed and validated for determination of Zaltoprofen in bulk and tablet dosage form. The estimation was carried out on Enable C18G (250 mm ×4.6 mm, 5 μm) column using Acetonitrile and Methanol in the ratio of 90:10 (v/v) as mobile phase. The flow rate was 1.0 ml/min and the effluent was monitored by UV detector at 331 nm. The retention time was 3.639 min and linearity was observed in the concentration range of 5-50 μg/ml. The percentage recovery was in good agreement with the labelled amount in the pharmaceutical formulations and the method was simple, precise and accurate for the determination of Zaltoprofen in bulk and pharmaceutical formulations.