Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2010 Vol: 2 Issue: 6

Development and validation of RP- HPLC method for estimation of Donepezil HCl from bulk and marketed dosage forms


A simple, fast and precise Reverse Phase High Perfo rmance Liquid Chromatographic ( RP- HPLC) method developed for the determination of Don epezil Hcl tablets, C 18 column 250mm x 4.6mm(l x d) in reverse phase isocratic mode of sep aration with mobile phase methanol : 0.02m phosphate buffer : Triethylamine (60:40:0.5)% v/v w ere used. The flow rate was 1ml/min. Linearity for Donepezil Hcl were in the range of 50 mcg/ml - 150mcg/ml. Amount found of Donepezil Hcl in Aricept 5mg, Aricept10mg, Dopezil 5mg tablets were 5.0072mg/tab, 10.01mg/tab and 5.01mg/tab respectively. Percentage recovery obtained was 100.53%, 100.24% and 100.34%. The proposed method is accurate, preci se, selective and rapid for the estimation of Donepezil Hcl in tablet dosage.