Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2014 Vol: 6 Issue: 6

Development and validation of analytical method for simultaneous estimation of amoxycillin trihydrate and probenecid in combined dosage form


A simple, fast and precise reverse phase high performance liquid chromatographic method was developed for the simultaneous estimation of Amoxycillin trihydrate and Probenecid in combined dosage form. The chromatographic separation was achieved on Zorbax RX-C18 (150x4.6) mm; 5μm column with an isocratic mixture of 0.05M potassium dihydrogen phosphate buffer pH 5.6 adjusted with a mixture of trifluoro acetic acid and tetrahydrofuran (0.5%): acetonitrile in the ratio of 86:14 v/v. The mobile phase was kept at a flow rate of 0.8ml/min with injection volume of 20μl and wavelength of detection 227nm at room temperature. The retention times for AMOX and PRO was found to be 1.85±0.1min and 6.90±0.1min, respectively. The linearity was obtained in the range of 50-250μg/ml for both Amoxycillin trihydrate and Probenecid with correlation coefficient 0.999 for both. On carrying out degradation studies it was found that products did not interfere with the detection of AMOX and PRO. The proposed method was found to be linear, accurate, precise, stable, robust and specific and was successfully applied for the determination of investigated drugs in combined dosage form.

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