Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2018 Vol: 10 Issue: 6

Development and Validation of Absorbance Correction Method for Simultaneous Estimation of Paracetamol, Ibuprofen and Dextromethorphan


In current study infrared spectroscopy was performed for identification of Paracetamol (PCM), Ibuprofen (IBU) and Dextromethorphan (DEX). Simultaneous spectrophotometric determination of Paracetamol, Ibuprofen and Dextromethorphan was carried out by absorbance correction method using UV visible double beam spectrophotometer. Validation of simultaneous spectrophotometric method was performed with different parameter like accuracy, precision, recovery and LOD and LOQ. For absorbance correction method 226 nm, 247 nm and 279 nm were selected as working wavelength because at 279 nm and 226 nm Dextromethorphan, at 226 nm Ibuprofen and at 226 nm, 247 nm 279 nm Paracetamol showed significant absorbance. Linearity of Paracetamol, Ibuprofen and Dextromethorphan was found to be 2-40 μg/mL, 2-50 μg/mL and 1-10 μg/mL respectively. The % mean, standard deviation (S.D.), relative standard deviation (%R.S.D.) and standard error (S.E.) were calculated. The % R.S.D. was less than 2% as required by USP and ICH guideline. Present study revealed that absorbance correction method was found to be simple, accurate and precise. The experimental work involved is very simple, it requires only measurement of absorbance at selected wavelength. Current study revealed development and validation of simple, accurate, precise, rapid, cost effective, reproducible and reliable UV spectrophotometric method for estimation of Paracetamol, Ibuprofen and Dextromethorphan in bulk and synthetic mixture.