Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2010 Vol: 2 Issue: 4

Development and validation of a UV spectrophotometric method for the estimation of torsemide in bulk and in tablet dosage form


A simple and cost effective spectrophotometric method described is based on use of 0.1N sodium hydroxide solution in which the drug is completely soluble, is used as a solvent. The drug has an absorption maximum at 290 nm and obeys Beer’s Lambert law in the concentration range 1– 25mg ml-1. The absorbance was found to increase linearly with increasing concentration of torsemide, which is corroborated by the calculated correlation coefficient value of 0.9999(n=6). The apparent molar absorptivity is1.896x103 L mol-1cm-1. The slope and intercept of the equation of the regression line are 3.4 ×10-2 and 11.2x10-2 respectively. The optimum experimental parameters for the reaction have been studied. The validity of the described procedure was assessed. Statistical analysis of the results has been carried out revealing high accuracy and good precision. The proposed method was successfully applied to the determination of torsemide in pharmaceutical formulations

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