Original Articles: 2013 Vol: 5 Issue: 5
Development and validation of a normal phase HPLC method for separation of anacardic acid isomers in cashew nut shell liquid
A simple normal phase HPLC method was developed and validated for the separation and determination of anacardic acid isomers present in cashew nut shell liquid. A Chiralpack, IA Chiral column (250 mm x 4.6 mm, packed with 5 microns) is used as stationary phase. An isocratic mode with mobile phase consists of 0.1% trifluroacetic acid in n- Hexane and IPA in ration of 1:1000:10 (v/v/v), at a flow rate of 1 mL/min. and the eluent was monitored at a wavelength of 210 nm. The result obtained from method validation showed a good agreement with the declared content. The method shows good linearity in the range of 0.06-0.24 mg/mL with correlation coefficient 0.9997-0.9999. Analytical recovery was between 99.0 -102.0 %. The proposed method successfully applied for separation of anacardic acid isomers i.e. saturated, monoene, diene and triene present in cashew nut shell liquid. The established normal phase HPLC method is precise, accurate and selective, because of its sensitivity and reproducibility.