Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2014 Vol: 6 Issue: 5

Determination of vitamin C and nicotinic acid content in black bean by capillary zone electrophoresis

Abstract

In this paper, capillary zone electrophoresis method was used for the determination of vitamin C and nicotinic acid content in black bean. 20 mmol/L borax solution and 20% acetonitrile concentration, 20kV voltage, 254 nm UV detection wavelength was chosen for electrophoretic analysis. Measured vitamin C and nicotinic acid content in black bean were 0.333 mg/g (RSD = 3.9%) (n = 5), 0.482 mg/g (RSD = 5.9%) (n = 5), respectively.

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