Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2018 Vol: 10 Issue: 12

Determination of Total Curcuminoid Content and Free Radical Scavenging Activity of Turmeric (curcuma longa linn.) Ethanol Extract with Different Soil type Sing dpph (1,1- diphenyl-2-picrylhydrazyl)


Natural antioxidants such as turmeric rhizome (Curcuma longa Linn.) Are known to have antioxidant activity against free radicals because they contain bioactive compounds in the form of curcumin. This study aims to determine the total levels of curcuminoids expressed as equivalent to curcumin (EK) and antioxidant activity using the DPPH method (1,1 diphenyl 2 picrylhydrazyl). Turmeric extract was obtained by using Soxhlet using ethanol solvent p.a. Curcuminoid identification in extracts and antioxidant activity by Thin Layer Chromatography (TLC) test. While the total levels of curcuminoids and antioxidant activity were determined by visible spectrophotometry. Quantitative analysis of total levels of curcuminoids and antioxidant activity was carried out by determining the correlation coefficient value of the linear regression equation obtained. The qualitative test results showed that ethanol extract of turmeric from Alfisol and Inceptisol soil types positively contained curcuminoid compounds and had antioxidant activity. The total curcuminoid content in C.longa L. ethanol extract from Alfisol soil was 11.24 ± 0.409% (EK) while from Inceptisol soil type was 5.87 ± 0.174% (EK). Standard antioxidant activity of curcumin, ethanol extract of C.longa L. from Alfisol and Inceptisol soil types ES50 values of were 6.17 μg / mL, 29.56 μg / mL, and 39.08 μg / mL, respectively. Based on the results of the determination of total levels of curcuminoid and antioxidant activity it can be concluded that the extract of ethanol of turmeric from Alfisol soil has a total level of curcuminoid and higher antioxidant activity compared to ethanol extract of turmeric from Inceptisol soil type.
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