This Study was done in Kenana Sugar Factory in the Republic of Sudan. In this study sugar content determined using High Performance Liquid Chromatography HPLC and Ultra violet UV. The samples of various sugar cane juices and bagasse was taken during the industrial processes. The losses of sugar in bagasse also determined using those methods. The results of this experiment were shown in tables and graphics. The main reasons of the high losses in Kenana factory had been determined as one of the results of this study