Histamine causes many risks to human. Therefore, histamine detection in milk is important for infants. Beside milk components can be the relation to allergy, lactic acid bacteria existing in milk may be a cause. In the study, Lactobacillus produced histamine too low to detect by changing the color of from yellow to purple. However, thin layer chromatography (TLC), high performance liquid chromatography (HPLC) were set up to detect histamine at the detection level of histamine around 10 μg/ml in milk. The study clarified the histmine detection of lactic acid bacteria and warned milk products supplied with lactic acid bacteria should be used as soon as preparation.