Laser-induced fluorescence (LIF) and Raman spectroscopy were used to measure serum of esophagus cancer patients and healthy people. Eighteen esophagus cancer patients and 21 healthy volunteers participated in this research. Raman peak intensities between different groups were detected and compared. Three parameters were selected as the standard for comparison. This preliminary results show that Raman spectroscopy has the potential to target esophagus cancer.