The aim of this investigation was to prepare, characterize and optimize the vinblastine sulphate loaded proniosomes for overall improvement in the physical stability and to prolong the release time in a controlled manner there by increasing its efficacy and to reduce its toxicity and to study the suitability of proniosomes as the carrier of drug. Proniosomes of vinblastine sulphate were prepared by slurry method. The formulations were characterized with respect to shape and surface morphology, entrapment efficiency, invitro drug release profile, and by cell line studies and stability under specific conditions. The formulated proniosomes were smoother indicating a thin and uniform coating over maltodextrin powder. The vesicular size of the optimized formulation showed the vesicular size of 250- 300nm. The evaluation of entrapment efficiency showed that it played a significant role by varying the concentration of cholesterol and Span, the highest entrapment efficiency was found in formulation F6 with 84.41±0.636%. Highest cumulative percent drug release was observed with formulation F5 with 97.13% in 48 h. The cell line study result of formulated proniosomes reveals that the drug was showing its efficacy for a 48 hours and the percentage cell line inhibition of optimized formulation was 2 times double when compared to marketed formulation. The results of investigation demonstrated that vinblastine sulphate loaded proniosomes offers an alternative colloidal carrier approach in increasing its physical stability. The results obtained for the present study clearly revealed that proniosomes containing vinblastine sulphate are capable of releasing their drug for the extended period of time there by increasing the efficacy of drug.