Original Articles: 2013 Vol: 5 Issue: 5
Decolorization of synthetic textile dyes using Pseudomonas putida
Abstract
Some of the synthetic dyes are recalcitrant compounds that are difficult to degrade by most conventional methods.
In this study, crystal violet, safranin and trypan blue were decolorized using Pseudomonas putida. The effect of pH,
temperature, dye concentration, inoculum volume static/agitated conditions and different carbon and nitrogen
sources was determined using One Factor At a Time (OFAT) approach to maximize the decolorization process. The
optimum conditions were found to be pH 7, at 30°C, dye concentration of 200 mg/L, 0.5mL of inoculum for crystal
violet and safranin whereas pH 7, at 40°C, dye concentration 100mg/L, inoculum volume 0.5 mL for trypan blue.
The bacteria showed higher decolorization in static compared to agitated condition. The decolorization efficiency
was higher when starch and ammonium sulphate were used supplements. On physiochemical parameter
optimization, crystal violet, safranin and trypan blue were degraded by 50%, 30% and 80% respectively.