The objective of the present study is to examine the antibacterial, antioxidant and anticancer activity of acetone and methanol tissue extracts of the marine gastropod Hemifusus pugilinus. Solvent extracts of H. pugilinus were tested for their inhibitory action against Escherichia coli, Salmonella typhi, Staphylococcus aureus and Streptococcus sp. by agar well diffusion method. Maximum zone of inhibition was exerted by methanol extract against S. typhi (21.36 ± 1.44 mm) at 150 μg/mL concentration. Antioxidant potential of extracts of H. pugilinus was evaluated using free radicals of 1,1-diphenyl-2-picrylhydrazyl, nitric oxide, superoxide anion and hydrogen peroxide. Methanol extract exhibited more efficient radical scavenging activity with IC50 concentration of 74.73 μg/mL towards nitric oxide. The cytotoxicity of the tissue extract was tested against MDA-MB-231 breast cancer cell line by MTT assay. Minimum cancer cell viability (20.08 ± 2.83%) was observed in methanol extract at 100 μg/mL concentration. The tissue extracts exhibited statistically significant (p<0.05) activity in all the bioassays. However, the standard drug exerted better activity than the tissue extracts. Preliminary qualitative analysis revealed that the crude extract has alkaloids, phenol, flavonoid, terpenoid, glycoside, sterols and fatty acids. From the present study, it has been concluded that tissue extract of H. pugililnus possess drug like bioactive compounds.