Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

Reach Us reach to JOCPR whatsapp-JOCPR +44 1625708989
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Original Articles: 2015 Vol: 7 Issue: 3

Cytokine secretion of human keratocytes following crosslinking/riboflavin-UVA photodynamic inactivation (PDI), in vitro


Crosslinking/Riboflavin-UVA photodynamic inactivation (PDI) is a potential treatment option in keratoconus and in therapy resistant infectious keratitis. We aimed to evaluate the impact of crosslinking/riboflavin-UVA-PDI on TGFβ1, FGFb, VEGF, HGF, KGF, IL-6 and IL-8 secretion of normal human keratocytes, in vitro. Primary human keratocytes were isolated by digestion in collagenase (1.0 mg/ml) from human corneal buttons, and cultured in DMEM/Ham's F12 medium supplemented with 10% FCS. Cell cultures underwent UVA-light illumination (8.0 mW/cm2) for 4.10 minutes during exposure to 0.1% concentration of riboflavin-5-phosphat. Five and 24 hours after crosslinking, cytokine concentration was determined in the culture supernatant using ELISA. Five hours after riboflavin-UVA-PDI, FGFb, VEGF and HGF secretion of keratocytes decreased significantly (p<0.01; p<0.05; p<0.01) and TGFβ1, IL-6 and IL-8 secretion remained unchanged. Twenty-four hours following riboflavin-UVA-PDI, FGFb and KGF secretion decreased (p<0.01; p<0.01) and IL-6 secretion increased significantly (p<0.01), whereas TGFβ1, VEGF and HGF secretion remained unchanged. Crosslinking/riboflavin-UVA-PDI decreases FGFb, VEGF and HGF secretion 5 hours and FGFb and KGF secretion 24 hours after treatment, while it increases IL-6 secretion of keratocytes after 24 hours. In the short term, riboflavin-UVA-PDI does not have an impact on TGFβ1 and IL-8 secretion of keratocytes, in vitro.

rtp slot demo