The objective of the present study was to investigate the effect of concomitant administration of trigonelline (TRIG) along with sitagliptin (SITA) on glucose level, behavior, oxidative stress and histological studies in nicotinamidestreptozotocin induced diabetic neuropathy in Wistar rats. Diabetes was induced by administration of streptozotocin (65 mg/kg i.p), 15 min after nicotinamide (110mg/kg) injection. After developing hyperglycemia, control and diabetic rats were randomly selected and divided into five groups (n=6). These includes, Group 1 was: nondiabetic, Group 2: diabetic control, Group 3: diabetic + TRIG (50 mg/kg), Group 4: diabetic + SITA (5 mg/kg), Group 5: diabetic + TRIG (50mg/kg) + SITA (5 mg/kg). TRIG, SITA and TRIG+SITA administered for 4 weeks. Serum glucose (SG) levels and body weights of animals were measured at every week of study period. Motor nerve conduction velocity and behavioral parameters were also screened at 0, 4th and at 8th weeks of study. Levels of SOD, MDA and histopathological study were carried out on the last day of study. Administration of these drugs resulting significant reduction in SG levels, decreasing lipid peroxidation and increase in the SOD levels. Histopathology examination of the sciatic nerve sections from TRIG+SITA treated rats showed absence of necrosis, mild congestion and swellings as compared to monotherapy. Study undertaken demonstrated that concomitant treatment of TRIG + SITA showed promising therapeutic effect against experimental diabetic neuropathy in rats than monotherapy.