Grape seed is one of proanthocyanidins sources where antioxidant activity of proanthocyanidins is 20 times greater than vitamin E and 50 times greater than vitamin C. Based on the findings, the aim of this research is to recognize the comparison of the most effective antioxidant activity of grape seed (Vitis vinifera L.) of red and green varieties with DPPH method. The principle in this research ranged from extraction by ethyl acetate followed by the process of determination of antioxidant activity assay with DPPH method in UV-Vis spectrophotometry. The results showed that antioxidant activity of green grape seed varieties is higher than red varieties with IC50 33.937 μg/mL and red varieties 60.947 μg/mL respectively.